3VZ6

Crystal Structure Analysis of the Mini-chaperonines, variant with Gly 184 replaced with Ile and Leu 185 replaced Val and Val 186 replaced with Leu.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-28
Detector	ADSC QUANTUM 4r
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	75.492, 79.900, 35.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.950 - 1.500
R-factor	0.268
Rwork	0.268
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kid
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	31276
Completeness [%]	90.0	90

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	288	0.1M Tris-HCl,1M NaCl, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 288K