3VYY
Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase A (DHX9)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.007 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 52.252, 78.529, 112.242 |
Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
Resolution | 37.010 - 2.900 |
R-factor | 0.22097 |
Rwork | 0.219 |
R-free | 0.26175 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1whq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.644 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 9511 | |
<I/σ(I)> | 27.7 | 5.8 |
Completeness [%] | 99.5 | 94.7 |
Redundancy | 3.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, CaAc2, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |