3VYI
Crystal Structure of a trimeric coiled-coil (I/I-type) assembly domain from the voltage-gated proton channel mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-07-22 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.273, 84.010, 88.393 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.114 - 2.305 |
R-factor | 0.2356 |
Rwork | 0.232 |
R-free | 0.29480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP (& PHASER) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Number of reflections | 27673 | ||
Completeness [%] | 99.1 | 92.4 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M AmSO4, 0.1M Tris-HCl (pH 8.5), 25% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |