3VYA
W32Y mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-02-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 44.119, 44.119, 178.590 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.20811 |
Rwork | 0.205 |
R-free | 0.27348 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1flm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.116 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.128 | 0.258 |
Number of reflections | 4587 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 15.3 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.05 | 293 | 100mM HEPES, 85%(v/v) 2-Methyl-2,4-pentanediol, 50%(w/v) polyethylene glycol 400, pH 8.05, VAPOR DIFFUSION, SITTING DROP, temperature 293K |