3VY5
N33Q mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2011-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.767, 83.993, 40.445 |
Unit cell angles | 90.00, 93.74, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.400 |
R-factor | 0.16694 |
Rwork | 0.166 |
R-free | 0.18397 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1flm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.165 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.059 | 0.279 |
Number of reflections | 48156 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG 6000, 0.1M Tris, 0.2M sodium acetate, 16.8% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |