3VXU

The complex between T36-5 TCR and HLA-A24 bound to HIV-1 Nef134-10(2F) peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-02
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 32
Unit cell lengths	73.162, 73.162, 415.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.350 - 2.700
Rwork	0.272
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vxt 3vxn
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge		0.344
Number of reflections	62839
<I/σ(I)>	12.6
Completeness [%]	92.2	80.5
Redundancy	5.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	10% PEG3350, 0.1M sodium nitrate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K