3VXO
HLA-A24 in complex with HIV-1 Nef134-10(2F)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-01 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 51.120, 66.382, 87.316 |
Unit cell angles | 112.30, 89.88, 90.01 |
Refinement procedure
Resolution | 50.000 - 2.610 |
R-factor | 0.1887 |
Rwork | 0.186 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vxn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.353 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.150 | 0.398 |
Number of reflections | 31267 | |
<I/σ(I)> | 10.6 | |
Completeness [%] | 96.4 | 93.4 |
Redundancy | 2.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 8000, 200mM ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |