3VXC

Crystal Structure of Xylobiose-BxlE complex from Streptomyces thermoviolaceus OPC-520

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS VII
Spacegroup name	P 21 21 2
Unit cell lengths	103.672, 163.157, 42.931
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.810 - 1.850
R-factor	0.17026
Rwork	0.168
R-free	0.21282
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	1.816
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.780
Rmerge	0.071
Number of reflections	69417
Completeness [%]	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.2	293	25% PEG 3350, 0.1M citrate, 15% glycerol, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	3.2	293	25% PEG 3350, 0.1M citrate, 15% glycerol, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K