3VX8
Crystal structure of Arabidopsis thaliana Atg7NTD-Atg3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 101.520, 132.683, 102.805 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.850 - 3.110 |
| R-factor | 0.2287 |
| Rwork | 0.226 |
| R-free | 0.27770 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.974 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.162 | 0.069 | 0.492 |
| Number of reflections | 25356 | ||
| <I/σ(I)> | 7 | ||
| Completeness [%] | 99.8 | 97.9 | 99.8 |
| Redundancy | 5.7 | 4.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 16% PEG 3350, 100mM Magnesium formate, 20mM Tris-HCl, 2mM DTT, NaCl, pH 8.0, vapor diffusion, sitting drop, temperature 293K |
