3VX0
Crystal Structure of alpha-amylase from Aspergillus oryzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-07-14 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.524, 65.619, 130.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.970 - 1.500 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.106 | 0.541 |
Number of reflections | 66313 | |
<I/σ(I)> | 7.7 | 3.6 |
Completeness [%] | 98.5 | 95.3 |
Redundancy | 5.7 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Oil-microbatch method | 5.8 | 293 | PEG 8000, CaCl2, pH 5.8, Oil-microbatch method, temperature 293K |