3VWX
Structural analysis of an epsilon-class glutathione S-transferase from housefly, Musca domestica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-01-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 87.548, 87.548, 131.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.820 - 1.800 |
| R-factor | 0.16532 |
| Rwork | 0.164 |
| R-free | 0.19052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jlv |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.719 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.800 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 102983 | |
| Completeness [%] | 98.3 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.2 M sodium citrate, 0.1 M HEPES-NaOH pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
