3VVX
Crystal Strucuture of RamR (Transcriptional Regurator of TetR Family) from Salmonella Typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-27 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.792, 77.594, 120.185 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.920 - 2.050 |
R-factor | 0.2363 |
Rwork | 0.234 |
R-free | 0.27070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.536 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 5.560 | 2.050 |
Rmerge | 0.055 | 0.023 | 0.299 |
Number of reflections | 24191 | ||
<I/σ(I)> | 21.9 | ||
Completeness [%] | 99.9 | 99.2 | 100 |
Redundancy | 26 | 20.4 | 28 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM MES, 20mM Tris-HCl, 75mM KCl, 2mM DTT, 5.88% PEG 20000, 50% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |