3VVR
Crystal structure of MATE in complex with MaD5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.521, 59.599, 68.335 |
| Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
| Resolution | 39.624 - 3.000 |
| R-factor | 0.2707 |
| Rwork | 0.267 |
| R-free | 0.30870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vvn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.726 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 3.000 | 7.460 |
| Rmerge | 0.236 | 0.074 |
| Number of reflections | 12863 | |
| <I/σ(I)> | 3.8 | |
| Completeness [%] | 99.1 | 97.1 |
| Redundancy | 3.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipdic cubic phase | 7 | 293 | 30% PEG 400, 100mM HEPES-NaOH, 100mM NaSCN, pH 7.0, lipdic cubic phase, temperature 293K |
