3VVO
Crystal structure of MATE in the bent conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-27 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.900 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.893, 58.835, 71.491 |
Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
Resolution | 31.663 - 2.504 |
R-factor | 0.2113 |
Rwork | 0.206 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.879 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.663 | 2.540 |
High resolution limit [Å] | 2.504 | 2.500 |
Rmerge | 0.216 | |
Number of reflections | 21175 | |
<I/σ(I)> | 15.2 | |
Completeness [%] | 95.9 | 98.8 |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | lipdic cubic phase | 6.5 | 293 | 32% PEG 400, 50mM MES-NaOH, 20mM CaCl2, 100mM NaSCN, pH 6.5, lipdic cubic phase, temperature 293K |