3VVO
Crystal structure of MATE in the bent conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-27 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.893, 58.835, 71.491 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 31.663 - 2.504 |
| R-factor | 0.2113 |
| Rwork | 0.206 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.879 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.663 | 2.540 |
| High resolution limit [Å] | 2.504 | 2.500 |
| Rmerge | 0.216 | |
| Number of reflections | 21175 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 95.9 | 98.8 |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | lipdic cubic phase | 6.5 | 293 | 32% PEG 400, 50mM MES-NaOH, 20mM CaCl2, 100mM NaSCN, pH 6.5, lipdic cubic phase, temperature 293K |
