3VVK
An M-like Reaction State of the azide-bound purple form of pharaonis halorhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.850, 97.900, 101.020 |
| Unit cell angles | 90.00, 128.53, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| Rwork | 0.211 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a7k |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.000 | 79.030 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.097 | 0.057 | 0.348 |
| Number of reflections | 48183 | ||
| <I/σ(I)> | 9.6 | 18.6 | 3.5 |
| Completeness [%] | 92.5 | 97.8 | 99.8 |
| Redundancy | 3.6 | 3.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 5 mg/ml nonyl glucoside, 2.7M ammonium sulfate, 0.16M NaCl, 0.1M HEPES, 0.04% NaN3 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
