3VVI
Crystal structure of the coiled-coil domain of the transient receptor potential channel from Gibberella zeae (TRPGz)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-27 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97909, 0.97944, 0.99159 |
| Spacegroup name | P 43 |
| Unit cell lengths | 35.290, 35.290, 120.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.950 - 1.250 |
| R-factor | 0.14903 |
| Rwork | 0.146 |
| R-free | 0.19991 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.068 | 0.582 |
| Number of reflections | 40607 | |
| <I/σ(I)> | 45.3 | 3.8 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 10.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 18-25% PEG3350, 0.2M sodium acetate, 0.1M HEPES, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
