3VUV
Crystal structure of the merozoite surface protein MSPDBL2 from P. falciparum bound to zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2718 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.690, 102.070, 38.245 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 37.442 - 2.114 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wau |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.442 | 20.000 | 2.240 |
| High resolution limit [Å] | 2.110 | 6.330 | 2.110 |
| Number of reflections | 15702 | ||
| Completeness [%] | 99.1 | 99.5 | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 20% PEG3350 0.2M sodium thiocyanate, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
