3VUP
Beta-1,4-mannanase from the common sea hare Aplysia kurodai
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-10-31 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.80 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 110.092, 114.098, 51.138 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.950 - 1.050 |
R-factor | 0.1377 |
Rwork | 0.137 |
R-free | 0.15650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.252 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.070 |
High resolution limit [Å] | 1.050 | 1.050 |
Number of reflections | 292160 | |
Completeness [%] | 98.5 | 93.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1M sodium sulfate, 100mM Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |