3VUO
Crystal structure of nontoxic nonhemagglutinin subcomponent (NTNHA) from clostridium botulinum serotype D strain 4947
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS VII |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 147.851, 147.851, 229.741 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.900 |
| R-factor | 0.23106 |
| Rwork | 0.227 |
| R-free | 0.30947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.752 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.080 |
| High resolution limit [Å] | 3.900 |
| Number of reflections | 25867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2M ammonium sulfate, 30%(w/v) poly-ethylene glycol monomethyl ether 2000, 0.1M sodium acetate trihydrate, pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
