3VU9
Crystal Structure of Psy3-Csm2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-25 |
| Detector | ADSC Quantum 270r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.560, 50.403, 76.350 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.750 |
| R-factor | 0.1847 |
| Rwork | 0.182 |
| R-free | 0.23119 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.280 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.048 | 0.485 |
| Number of reflections | 45389 | |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 90.0 | |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Hanging Drop Vapor Diffusion | 7 | 293 | 0.1M Tris-HCl, 30% Ethylene glycol(in case of Se-Met derivative, 25%), 5% polyethylene glycol, pH 7.0, Hanging Drop Vapor Diffusion, temperature 293K |
