3VU4

Crystal structure of Kluyvelomyces marxianus Hsv2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	90
Detector technology	CCD
Collection date	2012-05-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	140.270, 140.270, 251.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.980 - 2.600
R-factor	0.224
Rwork	0.224
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.062	0.556
Number of reflections	45676
<I/σ(I)>	17.4
Completeness [%]	99.9	99.6
Redundancy	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1M AcOH, 1.2M Ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
2