3VU2
Structure of the Starch Branching Enzyme I (BEI) complexed with maltopentaose from Oryza sativa L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.810, 66.560, 169.625 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 37.220 - 2.230 |
| R-factor | 0.22908 |
| Rwork | 0.226 |
| R-free | 0.28641 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.016 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 72156 |
| Completeness [%] | 88.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
