3VU1
Crystal structure of the C-terminal globular domain of oligosaccharyltransferase (PhAglB-L, O74088_PYRHO) from Pyrococcus horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.467, 94.844, 186.345 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.700 |
R-factor | 0.1735 |
Rwork | 0.171 |
R-free | 0.21469 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zag |
RMSD bond length | 0.011 |
RMSD bond angle | 1.494 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.064 | 0.294 |
Number of reflections | 41657 | |
<I/σ(I)> | 22.81 | 4 |
Completeness [%] | 99.3 | 89.8 |
Redundancy | 6.8 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15%(w/v) PEG3350, 0.2M sodium citrate, 0.1M bis-Tris propane-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |