3VTY

Crystal structure of MamA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	51.701, 101.315, 139.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.990 - 2.000
R-factor	0.1653
Rwork	0.163
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	1.734
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.990	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	49745
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	293	0.1M Sodium Acetate pH 4.4, 1.75M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K