3VTX
Crystal structure of MamA protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.457, 77.548, 77.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.980 - 1.750 |
| R-factor | 0.1637 |
| Rwork | 0.162 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.980 | 1.791 |
| High resolution limit [Å] | 1.745 | 1.740 |
| Number of reflections | 47254 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vappor diffusion, sitting drop | 4.4 | 293 | 0.1M BICINE pH 9, 2M MgCl, Vappor diffusion, sitting drop, temperature 293K |
