3VTN
The crystal structure of the C-terminal domain of Mu phage central spike - Pt derivative for MAD
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-30 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0717, 1.0721, 1.0539 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 47.171, 47.171, 72.114 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.060 - 1.750 |
R-factor | 0.27436 |
Rwork | 0.273 |
R-free | 0.30418 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.676 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.060 |
High resolution limit [Å] | 1.750 |
Number of reflections | 9825 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 21% PEG 8000, 0.1M acetate pH 4.5, 0.2M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |