3VTI
Crystal structure of HypE-HypF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.739, 119.101, 174.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.560 |
| R-factor | 0.20866 |
| Rwork | 0.206 |
| R-free | 0.25064 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3VTH (chains A and B) 2RB9 (chains C and D) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.077 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.560 | 2.600 |
| Rmerge | 0.075 | 0.538 |
| Number of reflections | 78733 | |
| <I/σ(I)> | 12.2 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 0.1M PIPES, 0.48M KCl, 9% PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
