3VT3

Crystal structures of rat VDR-LBD with R270L mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Collection date	2007-05-29
Wavelength(s)	0.97800
Spacegroup name	C 1 2 1
Unit cell lengths	153.883, 41.626, 41.956
Unit cell angles	90.00, 96.31, 90.00

Refinement procedure

Resolution	38.430 - 1.700
R-factor	0.202
Rwork	0.202
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zlc
RMSD bond length	0.006
RMSD bond angle	0.800
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	27435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris-HCl, sodium formate, PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K