3VSV
The complex structure of XylC with xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Collection date | 2011-03-10 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.779, 201.948, 100.105 |
| Unit cell angles | 90.00, 99.18, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.480 |
| Rwork | 0.150 |
| R-free | 0.16800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vst |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.071 | 0.496 |
| Number of reflections | 574432 | |
| <I/σ(I)> | 20.9 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 0.2M sodium citrate, 15-17%(w/v) polyethylene glycol 3350, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
