3VST
The complex structure of XylC with Tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.638, 202.237, 99.915 |
| Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
| Resolution | 24.930 - 1.750 |
| Rwork | 0.151 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet model |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.062 | 0.375 |
| Number of reflections | 346368 | |
| <I/σ(I)> | 19.1 | 2.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 0.2M sodium citrate, 15-17%(w/v) polyethylene glycol 3350, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
