3VSS
Microbacterium saccharophilum K-1 beta-fructofuranosidase catalytic domain complexed with fructose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.416, 71.979, 58.811 |
Unit cell angles | 90.00, 95.09, 90.00 |
Refinement procedure
Resolution | 39.560 - 1.970 |
R-factor | 0.17407 |
Rwork | 0.169 |
R-free | 0.21796 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vsr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.221 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.077 | 0.331 |
Number of reflections | 33971 | |
<I/σ(I)> | 25.4 | 4.6 |
Completeness [%] | 98.5 | 96.1 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM sodium acetate pH 5.5, 22.5% polyethylene glycol 8000, 300mM glycine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |