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3VSS

Microbacterium saccharophilum K-1 beta-fructofuranosidase catalytic domain complexed with fructose

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-6A
Synchrotron sitePhoton Factory
BeamlineBL-6A
Temperature [K]100
Detector technologyCCD
Collection date2008-05-18
DetectorADSC QUANTUM 4
Wavelength(s)1.0
Spacegroup nameP 1 21 1
Unit cell lengths58.416, 71.979, 58.811
Unit cell angles90.00, 95.09, 90.00
Refinement procedure
Resolution39.560 - 1.970
R-factor0.17407
Rwork0.169
R-free0.21796
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3vsr
RMSD bond length0.009
RMSD bond angle1.221
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.040
High resolution limit [Å]1.9701.970
Rmerge0.0770.331
Number of reflections33971
<I/σ(I)>25.44.6
Completeness [%]98.596.1
Redundancy3.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.5293100mM sodium acetate pH 5.5, 22.5% polyethylene glycol 8000, 300mM glycine, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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