3VSR
Microbacterium saccharophilum K-1 beta-fructofuranosidase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-09 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.723, 72.391, 58.709 |
| Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
| Resolution | 27.110 - 2.000 |
| R-factor | 0.19558 |
| Rwork | 0.193 |
| R-free | 0.24095 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pt2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.253 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.269 |
| Number of reflections | 31999 | |
| <I/σ(I)> | 36.4 | 9 |
| Completeness [%] | 98.4 | 95 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM sodium acetate pH 5.5, 22.5% polyethylene glycol 8000, 300mM glycine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
