3VSE
Crystal structure of methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9798 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.485, 91.573, 102.631 |
Unit cell angles | 90.00, 94.03, 90.00 |
Refinement procedure
Resolution | 34.126 - 2.099 |
R-factor | 0.204 |
Rwork | 0.203 |
R-free | 0.22970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.449 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX ((phenix.refine: dev_1021)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.099 | 2.100 |
Number of reflections | 103003 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 100mM sodium acetate pH4.5, 40 %(v/v) ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |