3VSC
Crystal Structure of the K127A Mutant of O-Phosphoserine Sulfhydrylase Complexed with External Schiff Base of Pyridoxal 5'-Phosphate with O-Phospho-L-Serine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.90000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.676, 74.676, 276.115 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.070 |
R-factor | 0.1716 |
Rwork | 0.169 |
R-free | 0.22730 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.026 |
RMSD bond angle | 2.261 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.5.0109) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.070 | 4.460 | 2.070 |
Rmerge | 0.079 | 0.043 | 0.334 |
Number of reflections | 48366 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.2 | 99.2 | 96.8 |
Redundancy | 8 | 9.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.1M HEPES sodium pH 7.5, 25% 2-propanol, 10% PEG 4000, 5mM O-phospho-L-serine, 5mM 2-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 296K |