3VS8
Crystal structure of type III PKS ArsC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.318, 143.388, 129.623 |
| Unit cell angles | 90.00, 110.33, 90.00 |
Refinement procedure
| Resolution | 37.570 - 1.760 |
| R-factor | 0.18513 |
| Rwork | 0.183 |
| R-free | 0.23014 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 345492 | |
| Completeness [%] | 97.2 | 80.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M imidazole-HCl, 0.15M calcium acetate, 12.5% polyethylene glycol 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
