3VRS
Crystal structure of fluoride riboswitch, soaked in Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.7712 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 57.846, 77.203, 42.094 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.779 - 2.603 |
R-factor | 0.2238 |
Rwork | 0.220 |
R-free | 0.26170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4enb |
RMSD bond length | 0.028 |
RMSD bond angle | 0.884 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.081 |
Number of reflections | 6020 |
<I/σ(I)> | 11 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1 M sodium cacodylate, pH 7.0, 20 mM spermine, 160 mM potassium chloride, 50 mM manganese chloride, 20% MPD, VAPOR DIFFUSION, temperature 293K |