3VRL
Crystal structure of BMJ4 p24 capsid protein in complex with A10F9 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.109, 124.058, 150.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.020 - 3.200 |
| R-factor | 0.21484 |
| Rwork | 0.211 |
| R-free | 0.29189 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1E6J (for FAB chains) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.127 | 0.448 |
| Number of reflections | 24996 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, NaBr, NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
