3VRH

Crystal structure of ph0300

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	70
Detector technology	CCD
Collection date	2010-01-01
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	70.071, 70.071, 128.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.236 - 2.100
R-factor	0.208
Rwork	0.206
R-free	0.24430
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Number of reflections	19923
Completeness [%]	99.9	99.8	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	12% PEG 3350, 0.2 M NaCl, 10% N,N-dimethylformamide, 0.1 M Bicine-NaOH, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 293K