3VRF
The crystal structure of hemoglobin from woolly mammoth in the carbonmonoxy forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.486, 61.790, 53.570 |
| Unit cell angles | 90.00, 110.35, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.550 |
| R-factor | 0.17243 |
| Rwork | 0.171 |
| R-free | 0.19926 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dn3 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.050 | 0.187 |
| Number of reflections | 47308 | |
| Completeness [%] | 97.4 | 83.2 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 7.5 | 293 | Sodium/potassium phosphate, pH 7.5, BATCH, temperature 293K, temperature 293.0K |
