3VRE
The crystal structure of hemoglobin from woolly mammoth in the deoxy form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.640, 59.080, 62.100 |
| Unit cell angles | 96.72, 114.36, 113.86 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.20632 |
| Rwork | 0.203 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.359 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.510 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.098 | 0.327 |
| Number of reflections | 28619 | |
| Completeness [%] | 94.4 | 93.9 |
| Redundancy | 2.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 6.5 | 293 | Ammonium sulfate, Ammonium phosphate, pH 6.5, Batch, temperature 293K, BATCH, temperature 293.0K |
