3VRE
The crystal structure of hemoglobin from woolly mammoth in the deoxy form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-01-24 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 53.640, 59.080, 62.100 |
Unit cell angles | 96.72, 114.36, 113.86 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.20632 |
Rwork | 0.203 |
R-free | 0.26850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.359 |
Data reduction software | MOSFLM |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.510 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.098 | 0.327 |
Number of reflections | 28619 | |
Completeness [%] | 94.4 | 93.9 |
Redundancy | 2.6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 6.5 | 293 | Ammonium sulfate, Ammonium phosphate, pH 6.5, Batch, temperature 293K, BATCH, temperature 293.0K |