3VRC
Crystal structure of cytochrome c' from Thermochromatium tepidum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.8 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.310, 57.189, 54.225 |
Unit cell angles | 90.00, 94.81, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.000 |
R-factor | 0.1304 |
Rwork | 0.129 |
R-free | 0.15340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bbh |
RMSD bond length | 0.021 |
RMSD bond angle | 2.482 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.020 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.040 | 0.210 |
Number of reflections | 127655 | |
<I/σ(I)> | 42.6 | 2.6 |
Completeness [%] | 94.3 | 70.2 |
Redundancy | 3.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 30% PEG 400, 0.1M cadmium chloride, 0.1M sodium acetate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |