3VR6
Crystal structure of AMP-PNP bound Enterococcus hirae V1-ATPase [bV1]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 126.149, 127.416, 225.252 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.580 - 2.680 |
| R-factor | 0.18481 |
| Rwork | 0.182 |
| R-free | 0.24618 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vr4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.790 |
| High resolution limit [Å] | 2.680 | 2.690 |
| Rmerge | 0.154 | 0.767 |
| Number of reflections | 97250 | |
| <I/σ(I)> | 9.57 | 1.76 |
| Completeness [%] | 96.1 | 89.2 |
| Redundancy | 5.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 20% PEG 3350, 0.1M Bis-Tris propane, 0.2M sodium fluoride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
