3VR2
Crystal structure of nucleotide-free A3B3 complex from Enterococcus hirae V-ATPase [eA3B3]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.800, 121.500, 128.260 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 44.180 - 2.800 |
| R-factor | 0.20061 |
| Rwork | 0.198 |
| R-free | 0.24293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gqb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.333 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.180 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.068 | 0.349 |
| Number of reflections | 91463 | |
| <I/σ(I)> | 11.6 | 3.5 |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 24% PEG 3350, 0.1M Tris, 0.2M Ammonium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
