3VR0

Crystal structure of Pyrococcus furiosus PbaB, an archaeal proteasome activator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-24
Detector	RAYONIX MX225HE
Wavelength(s)	0.9000
Spacegroup name	C 2 2 21
Unit cell lengths	115.600, 156.400, 153.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.198
Rwork	0.196
R-free	0.23700
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.569
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.068	0.353
Number of reflections	69614
<I/σ(I)>	50.3	8.2
Completeness [%]	99.7	100
Redundancy	7.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	10% PEG 10000, 0.1M Bis-Tris, 50mM sodium acetate, 5mM NaAuCl4, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K