3VQR
Structure of a dye-linked L-proline dehydrogenase mutant from the aerobic hyperthermophilic archaeon, Aeropyrum pernix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.987, 70.570, 73.092 |
| Unit cell angles | 71.65, 70.85, 70.15 |
Refinement procedure
| Resolution | 24.200 - 2.010 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3axb |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 52556 | |
| Completeness [%] | 99.9 | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 6.5 | 293 | PEG8000, Mg acetate, Mes, pH 6.5, sitting drop, temperature 293K |
