3VQK
Small heat shock protein hsp14.0 of wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 117.428, 117.428, 115.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.130 - 4.500 |
| R-factor | 0.34 |
| Rwork | 0.333 |
| R-free | 0.40400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aab |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 4122 | |
| <I/σ(I)> | 42.6 | 2.7 |
| Completeness [%] | 87.2 | 76.1 |
| Redundancy | 3.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 10% PEG2000MME, 4% glycerol, 4% sucrose, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
