3VPY
Crystal structure of Arabidopsis DDL FHA domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.564, 48.709, 54.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.370 - 1.700 |
| R-factor | 0.19204 |
| Rwork | 0.188 |
| R-free | 0.23107 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.370 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.064 | 0.521 |
| Number of reflections | 13269 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG, HEPES, AS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
