3VPX

Crystal structure of leucine dehydrogenase from a psychrophilic bacterium Sporosarcina psychrophila.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	I 4
Unit cell lengths	135.589, 135.589, 123.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.780 - 2.550
Rwork	0.243
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1leh
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.044	0.308
Number of reflections	35953
<I/σ(I)>	49.3	7.5
Completeness [%]	99.2	98.3
Redundancy	6.9	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.05M trisodium citrate dehydrate, 0.05M lithium sulfate, 0.75M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K