3VPT

Crystal structure of Bombyx mori sigma-class glutathione transferase in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 65 2 2
Unit cell lengths	56.128, 56.128, 209.893
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.100 - 1.900
R-factor	0.206
Rwork	0.204
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.661
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.100
High resolution limit [Å]	1.900	1.900
Number of reflections	22394
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		0.1M HEPES, 30% (w/v) PEG 400, 20% (v/v) 1,2-propanediol, pH 7.5, VAPOR DIFFUSION, SITTING DROP