3VPR
Crystal Structure of a TetR Family Transcriptional Regulator PfmR from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-05 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.681, 57.584, 133.447 |
| Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
| Resolution | 28.810 - 2.270 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.081 | 0.263 |
| Number of reflections | 35016 | |
| <I/σ(I)> | 18.6 | 7.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 100mM Phosphate-citrate, 40% v/v PEG 300, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
